[ RadSafe ] LSC Method Assistance Needed - CO2

[ROY HERREN]

Dave,

    If I remember correctly, the Helicobacter pylori tests are performed using Mylar balloons.  Take a look at the photographs at the following website, http://www.kchealthcare.com/docs/U4791_PYtest_Broch.pdf

For an old procedure see http://interactive.snm.org/docs/pg_ch07_0403.pdf 

Roy Herren 




________________________________
From: "neildm at id.doe.gov" <neildm at id.doe.gov>
To: Daren.Perrero at illinois.gov; blreider at aol.com; radsafe at radlab.nl
Sent: Thu, January 7, 2010 4:25:14 PM
Subject: RE: [ RadSafe ] LSC Method Assistance Needed - CO2

I just noticed something about the process outlined in the request.
Perhaps I am making an assumption, but use of a rubber balloon for
collection of a sample analyzed for CO2 has the confounding factor that
CO2 will dissolve through rubber and escape, or so I understood. 

Wrong?  Not Applicable? 

Just a comment.

Dave Neil

-----Original Message-----
From: radsafe-bounces at radlab.nl [mailto:radsafe-bounces at radlab.nl] On
Behalf Of Perrero, Daren
Sent: Thursday, January 07, 2010 3:27 PM
To: blreider at aol.com; radsafe at radlab.nl
Subject: RE: [ RadSafe ] LSC Method Assistance Needed - CO2

In a previous life in a land far far away, we conducted similar tests in
the same manner on respired air for the presence of H-3.  Our method
simply involved passing the respired air through several loops of super
cooled tubing immersed in liquid nitrogen.  Thus we were able to avoid
'color quenching' and nasty issues associated with other bodily fluids
since the collection/extraction solutions we gathered were essentially
all water.  After that we were able to pick and choose our scintillation
cocktails based on their ability to deal with aqueous solutions for
maximum efficiency.

The opinions expresses are mine, all mine.....
I'm with the government and I'm here to help you (ack!)
daren.perrero(a)Illinois.gov

-----Original Message-----
From: radsafe-bounces at radlab.nl [mailto:radsafe-bounces at radlab.nl] On
Behalf Of blreider at aol.com
Sent: Wednesday, January 06, 2010 7:27 PM
To: radsafe at radlab.nl
Subject: [ RadSafe ] LSC Method Assistance Needed - CO2



One of my friends, a new HP, asked me if I could assist him in finding a
better cocktail for his SC work.  He writes:  "Our breath bioassay
monitors 14CO2.  So we have a solution that we make, and we bubble the
air from the balloons in the solution to trap the co2 and count it on
our LSC.  This has been done for a long while, but the mixture is made
up of some nasty stuff, so I just want to see if we can do better." 

The method they use a toluene based cocktail:  "solution that contains
toluene,sodium hyrdroxide, trimethylbenzene, and methyl glycol."
It has been a long time since I did LSC so if you have any experience
and advice I am sure he will appreciate it.
Thanks in advance.
Barbara Reider, CHP 

It has been a long time since I did LSC so if you have any experience
and advice I am sure he will appreciate it.
Thanks in advance.
Barbara Reider, CHP
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