Re: Voltility of S35 compounds

[Stan <swilson@emory.edu>]

Cell - labelling procedures using S35 amino acids (especially the 
incubation stage) are notorious for releasing a volatile degradation 
product. The exact chemical is not known to me, several have been
 suggested. The manufacturer's and users note that up to 0.1% is 
volatalized and suggest that stock vials should be opened only under a 
functioning fume hood, and that the incubator have an activated charcoal trap
placed within, as well as a smear survey after the incubation.
The trap can be a mat or just an open beaker with 40-50 cc of
charcoal.

Hope this helps. I have copies of some useful articles if you are
 interested.

 On Wed, 11 Oct 1995, Denis J. Rinkacs Jr. wrote:

> Hi,
> 
> One of my Principal Investigators asked if there is a problem with
> inhalation of S35 under the following conditions:
> 
> Experiment:  charging of native tRNAs using S35 cycteine as tracer,
> extract of E. coli (S100 fraction) as source of charging enzymes, yeast
> tRNAs added.  Incubation for 15 minutes at 37 C required.
> 
> This experiment would be difficult by not impossible to be performed in a hood.
> 
> Has anyone had experiance with this type experiment?  The PI is worried
> about exposure to her undergrads.
> 
> Thanks for your help.
> 
> Denny
> Carnegie Mellon University
> dr4i@andrew.cmu.edu
> 412-268-3221
>