Re: Blood Irradiation for GVHD

["Björn Cedervall" <bjorn_cedervall@hotmail.com>]

I doubt very strongly that you have _any_ surviving lymphocytes after a 
dose of 30 Gy (based on my own experimental work and also other 
informations) - a dose of 30 Gy should produce the order of 1500 DNA 
double-strand breaks (DSBs) per cell. In addition to the large number of 
DSBs, lymphocytes are more sensitive to ionizing radiation than for 
instance solid tissue cells. For red blood cells which have no DNA, I 
would guess it is possible that they could remain functionally from an 
oxygen transfer point of view. Their membranes may become "shaky" after 
30 Gy but I wouldn't be surprised if they still make it. I would welcome 
other experiences or opinions.

bjorn_cedervall@hotmail.com
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>From: "Carbaugh, Eugene H" <gene.carbaugh@pnl.gov>
>Date: Tue, 16 Mar 1999 11:59:02 -0600 (CST)
>
>If healthy blood is irradiated to prevent Graft vs.
>Host Disease, what is the impact on the healthy cells in the blood.  

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