Use of fume hood for S35?

[Jennifer Ehlert <Jennifer.A.Ehlert@cmich.edu>]

I am the RSO for a very small radiation program, so radiation is just one
of the safety areas that I cover.  There is currently one isotope lab that
is set up for use with P32, and the researcher would also like to use S35.
I am in need of guidance for S-35 usage as follows:

Compound:  S35-dATP
Source:  Amersham, Pharmacia Biotech
Amount per order:  250 uCi
Form:  Redivue stabilized and colored aqueous solution containing 5 mM
2-mercaptoethanol
Used for DNA sequencing:  Manual double-stranded DNA sequencing (Sanger
ddNTP method) will be performed using the Sequenase 2.0 kit for Amersham
Pharmacia.  Briefly, (1) DNA and primer will be denatured and neutralized,
(2) DNA will be precipitated and resuspended in reaction buffer and
single-stranded binding protein, (3) DTT, dNTPs, S35-dATP, and polymerase
will be added and incubated on a heat block at 37 C for 5 minutes, (4)
termination mix will be added to each tube or well and incubated another 10
minutes at 37 C, (5) stop solution will be added and the reactions will be
loaded and run on polyacrylamide sequencing gels (or stored at -20 C until
needed), (6) gels will be transferred to filter paper, covered with palstic
wrap, and autoradiographed.  The heated incubations will be done in a
chemical fume hood.

My questions are as follows:
1 - What form of S35 will be airborne from the heating block inside the hood?
2 - Do I need to filter/trap this in some way?
3 - If I need to filter/trap the radioactivity, what do you recommend?

Thanks in advance for your consideration!
Jennifer Ehlert
Safety Coordinator, College of Science and Technology
Central Michigan University
236 IET
Mount Pleasant, MI  48859
phone (517)774-4189
fax (517)774-1874
pager (517)612-1255
Jennifer.A.Ehlert@cmich.edu
************************************************************************
The RADSAFE Frequently Asked Questions list, archives and subscription
information can be accessed at http://www.ehs.uiuc.edu/~rad/radsafe.html