Andrew Karam, CHP
Andrew_Karam@URMC.Rochester.edu
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Anal Chem 1999 Oct 1;71(19):4423-6
Detection of low dose radiation induced DNA damage
using temperature differential fluorescence assay.
Rogers KR, Apostol A,
Madsen SJ, Spencer CW
A rapid and sensitive fluorescence assay for
radiation-induced DNA damage is reported. Changes in temperature-induced strand
separation in both calf thymus DNA and plasmid DNA (puc 19 plasmid from
Escherichia coli) were measured after exposure to low doses of radiation.
Exposures of between 0.004 and 1 Gy were measured with doses as low as 0.008 Gy
yielding significant responses. The double-strand, sensitive dye PicoGreen was
used as an indicator of DNA denaturation. Calibration plots indicate that
fluorescence changes corresponding to amounts as low as 1 ng of double stranded
DNA (10(6) copies for plasmid puc 19) are detected by this
method.