RE: S-35 contamination

["Allen, Tim" <TAllen@JAGUAR.MIDDLEBURY.EDU>]

Hi,



You have to be careful covering the exhaust hole of a centrifuge - some

students set a stryofoam rack on top of one of ours to reduce the noise, and

it caused major problems.  The rotor was pulled down somehow, and the

bottoms of the tubes were ground off against the inside of the centrifuge,

causing a total loss of sample.



Be sure to put in plenty of pinholes, and test it with non-radioactive

materials first, or you could be in for some major cleanup!



I would think screw top tubes with O-rings would be a better bet - that's

what we use for RNA isolation in a bead beater, and I've never seen one

leak.



Good luck!



		Tim Allen

		Senior Laboratory Technician / Radiation Safety Technician

		601A Bicentennial Hall

		Middlebury College

		Middlebury, VT 05753

		Phone: (802)443-5729

            Fax: (802) 443-2072





> ----------

> From: 	Thomas J Savin 

> Reply To: 	Thomas J Savin 

> Sent: 	Thursday, August 23, 2001 8:17 PM

> To: 	Andrew Mattox; radsafe@list.vanderbilt.edu

> Subject: 	Re: S-35 contamination

> 

> Hey everyone:

> 

> We never liked using mineral oil - of course there are numerous ways to

> keep solutions contained. I take it that the problem of which we speak is

> for lab top centrifugation.  In reality we had a dedicated centrifuge

> (eppendorf microfuge)in a rad hood.  Of course there was some

> contamination, but it was controlled.  It never got into the filters in

> the hood.  We would always do swipes and have a sheet of tyvek on top of

> the exhaust (hole on top of the centrifuge)with small pin holes to

> eliminate exhaust.  Heat transfer was determined to be of little

> consequence for our protien, DNA and RNA isolations due to the cooling

> effect of the negative air pressure - materials of interest were stable in

> there respective solutions @ air conditioned conditions  (DOE had great

> Air conditioning).  Hope this helps - for particulars please respond to me

> directly - this probably is of little interest to most on radsafe

> 

> Enjoy - Tom

> 

> ---

> Tom Savin

> 

> On Thu, 23 Aug 2001 16:24:46  

>  Andrew Mattox wrote:

> >Not sure about current availability but Research Products International

> (847-635-7330) has offered

> >disposable liners for Eppendorf 5414 and 5415 microfuges, ask for

> Microliner Spill Guards.

> >

> >Srebro wrote:

> >

> >>   Hi

> >>

> >>    I have a problem of contaminating centrifuge in one of our labs.

> >>   In the stage of separation between the glass beads and the extracted

> >> protein

> >>   they use an epentorff vial inside a vial and can't seal it so the

> result

> >> is

> >>   centrifuge contamination with S-35. Is any one using this procedure??

> >>    How you avoid contamination? Is there a different technique?

> >>

> >>

> Thanks, Rafi Srebro

> >>

> RSO Ben-Gurion University

> >>  srbro@bgumail.bgu.ac.il

> >>

> >>

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