RE: Tissue counting

["Akbarzadeh, Mansour" <AkbarzM@wipp.carlsbad.nm.us>]

                       One of the common methods used is freeze- drying
technique. In this method, using aetone and dry-ice mixture bath, The
sample is frozen in a closed system under vacuum and moisture containing
tritum is collected in a "finger separator"  (a long test tube attached
to the apparatus).It hs been a long time that I have done this method
but it works for both urine and tissue samples. The cocktail used is
usually "Optima Gold".I think, I still have a detailed procedure and I
will fax it to you as soon as I find it.       


>  ----------
>From: 	Andrew Buchan[SMTP:Andrew.Buchan@ehs.ucsb.edu]
>Reply To: 	radsafe@romulus.ehs.uiuc.edu
>Sent: 	Friday, October 09, 1998 10:53 AM
>To: 	Multiple recipients of list
>Subject: 	Tissue counting
>
>Hello,
>
>We are going to be counting some tissue samples from some mice that 
>have been injected with tritiated thymidine. 
>
>We are looking for recomendations on cocktails and sample prep of brain 
>tissue, liver tissue and perhaps a urine sample.
>
>Thank you in advance 
>
>Andrew
>
>
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