Re: Blood Irradiation for GVHD

["Björn Cedervall" <bjorn_cedervall@hotmail.com>]

Comment to my previous message: I was somewhat high with the DSBs - I 
think 1100 induced DSBs per cell after 30 Gy is a closer number in this 
case (the main point is still the same - that the white blood cells 
_all_ die following a dose of 30 Gy). I have, however, been much higher 
(more than 2000 DSBs for 30 Gy) for two squamous cell carcinoma lines. I 
repeated those (latter) experiments and came consistently to a higher 
value - the reason for this is unknown - I speculate that it may be 
relating to either compactness of the chromatin or level of thiols. But 
for this context - lymphocytes - and most other mammalian cells (I have 
looked at about 30 different cell lines from rodents and humans) it 
seems like about 35 to 40 DSBs/cell,Gy is a more reasonable value.

bjorn_cedervall@hotmail.com
----
>I doubt very strongly that you have _any_ surviving lymphocytes >after 
a dose of 30 Gy (based on my own experimental work and also >other 
informations) - a dose of 30 Gy should produce the order of >1500 DNA  
double-strand breaks (DSBs) per cell. 


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