Re: Irradiation of mouse thymus

["Otto G. Raabe" <ograabe@ucdavis.edu>]

December 21, 1999
Davis, CA

We have irradiated target organs in mice by placing them inside a 50 mL
centrifuge tube having a screw cap and a round ventilation hole at the
bottom of the tube. The mouse automatically puts its nose into the hole. We
then place the tube within a thick-walled (specially milled) lead cylinder
and put the whole assembly into the irradiator. You can calculate the
appropriate lead thickness depending on the gamma absorption that you
desire. An aperture on the lead shield will allow irradiation of the
appropriate portion of the mouse. More than the target organ is irradiated,
but the bone marrow and most of the body is shielded.

Otto

		*****************************************************
		Prof. Otto G. Raabe, Ph.D., CHP
              Institute of Toxicology & Environmental Health (ITEH)
		   (Street address: Building 3792, Old Davis Road)
		University of California, Davis, CA 95616
		Phone: 530-752-7754  FAX: 530-758-6140
		E-mail ograabe@ucdavis.edu
              *****************************************************
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