Re: Gamma Dose to cell sterilization

["Tom Savin" <lexicon@hotbot.com>]

 Just curious, but why give the extracts a dose of radiation? This will potentially mess up the components in the extract (via X-linking etc.)to the point they are useless for experimentation. A simple 20 ml syringe and a cheap (a couple of bucks)0.2 micron filter, attached to the syringe, will sterilize your 20 ml of extract. 

                         Hope this helps,

                              Tom
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On Fri, 5 May 2000 10:52:48    Mr Jo wrote:
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>Our researcher has chicken embryo cell extracts (dead cells). He wants to sterilize them using gamma (Cs-137) irradiator.  The sample has about 20 ml volume in liquid medium and it is in a plastic vial. I looked at doses required in food irradiation for meat, poultry, and fish and it was 0.1-10 kGy (wide range).
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>What dose shall I recommend? Any suggestions will be appreciated.
>
>Thank you and have a good weekend. M. Jo
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